R&D Systems™ Recombinant Human Wnt-3a Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.

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Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Gene ID (Entrez): 89780
• Molecular Weight (g/mol): 37.4 kDa
• Name: Wnt-3a
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human Wnt-3a protein Ser19-Lys352
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 75%, by SDS-PAGE with silver staining.