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R&D Systems™ Recombinant MERS-CoV His6 Papain-like Protease Protein
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Quantity:
50 μg
Description
Recombinant MERS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rh110 (U-555) substrate and 100-500 nM when using tetraubiquitin substrate.
Specifications
Specifications
| Accession Number | K9N638 |
| For Use With (Application) | Bioactivity |
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT |
| Molecular Weight (g/mol) | M.W.-theoretical: 37 kDa |
| Quantity | 50 μg |
| Source | E. coli-derived mers-cov Papain-like Protease protein Leu1541 - Ser1859 |
| Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 100 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening. |
| Conjugate | Unconjugated |
| Purity or Quality Grade | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain. |
| Protein | Papain-like Protease |
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