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R&D Systems™ Recombinant MERS-CoV His6 Papain-like Protease Protein

Catalog No. E609050
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50 μg
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E609050 50 μg
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Description

Carrier Free Recombinant Protein

Recombinant MERS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rh110 (U-555) substrate and 100-500 nM when using tetraubiquitin substrate.

Specifications

Accession Number K9N638
For Use With (Application) Bioactivity
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Molecular Weight (g/mol) M.W.-theoretical: 37 kDa
Quantity 50 μg
Source E. coli-derived mers-cov Papain-like Protease protein Leu1541 - Ser1859
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 100 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Conjugate Unconjugated
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein Papain-like Protease
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