R&D Systems™ Recombinant Mouse 4-1BB/TNFRSF9 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Measured by its ability to block 4-1BBL-induced IL-2 secretion by mouse T cells. The ED50 for this effect is 0.3-1 μg/mL in the presence of 0.4 μg/mL of recombinant mouse 4-1BBL. Measured by its ability to bind recombinant mouse 4-1BB Ligand in a functional ELISA.

Specifications

• Accession Number: P20334
• For Use With (Application): Bioactivity, Binding Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3604
• Molecular Weight (g/mol): M.W. (Observed): 62-66 kDa, reducing conditions; M.W. (theoretical): 44.3 kDa (monomer)
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse 4-1 BB/TNFRSF9/CD137 protein Mouse 4-1 BB (Val24-Leu187) (N-terminus) Accession # P20334 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Tnfrsf9
• Product Type: 4-1BB/TNFRSF9/CD137 Fc Chimera
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: 4-1 BB/TNFRSF9/CD137