R&D Systems™ Recombinant Mouse BID (Caspase-8-cleaved) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria. The typical EC50 for cytochrome c releasing activity is <100 nM. Cytochrome c is quantified using the Rat/Mouse Cytochrome c Quantikine™ ELISA Kit (Catalog # MCTC0 ). The EC50 for the desired application should be determined. Uncleaved Recombinant Mouse BID is available (Catalog # 860-MB ).

Specifications

• Accession Number: P70444
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES-KOH and KCl.
• Gene ID (Entrez): 637
• Molecular Weight (g/mol): M.W. (Observed): 7 kDa and 15 kDa, under reducing and non-reducing conditions; M.W. (theoretical): 7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment)
• Name: BID Caspase-8-cleaved
• Quantity: 50 μg
• Source: E. coli-derived mouse BID protein Met1-Asp195
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Gene Symbol: Bid
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: BID