R&D Systems™ Recombinant Mouse BTN1A1/Butyrophilin Protein

Description

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Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.

Specifications

• Accession Number: Q62556
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 696
• Molecular Weight (g/mol): M.W. (Observed): 34-42 kDa, reducing conditions; M.W. (theoretical): 25 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse BTN1A1/Butyrophilin protein Ala27-Trp247, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: BTBTNbK14H9.2 (butyrophilin, subfamily 1, member A1), BTN, BTN1A1, butyrophilin subfamily 1 member A1, butyrophilin, subfamily 1, member A1
• Common Name: BTN1A1/Butyrophilin
• Gene Symbol: Btn1a1
• Biological Activity: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is typically 0.3-1.5μg/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Expression System: Mouse myeloma cell line, NS0-derived Ala27-Trp247, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: BTN1A1/Butyrophilin