R&D Systems™ Recombinant Mouse CD28 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.3-1.2 μg/mL when stimulated with 1 μg/mL recombinant human B7-1 Fc Chimera in the presence of PHA.

Specifications

• Accession Number: P31041
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 940
• Molecular Weight (g/mol): M.W. (Observed): 60 kDa, reducing conditions; M.W. (theoretical): 52 kDa (monomer)
• Name: CD28 Fc Chimera
• Quantity: 200 μg
• Source: Mouse myeloma cell line, NS0-derived mouse CD28 protein Mouse CD28 (Lys21-Lys149) (N-terminus) Accession # P31041 DMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Cd28
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 400 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: CD28