R&D Systems™ Recombinant Mouse CD8 alpha Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse CD8 alpha Protein is derived from NS0. The Recombinant Mouse CD8 alpha Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P01731
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 925
• Molecular Weight (g/mol): Observed M.W.: 32-41kDa, reducing conditions, Theoretical M.W.: 20kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse CD8 protein Lys28-Tyr196,with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: CD8
• Gene Symbol: Cd8a
• Biological Activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 1-5μg/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Lys28-Tyr196, with a C-terminal 10-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining