R&D Systems™ Recombinant Mouse Complement Factor D Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse Complement Factor D Protein is derived from NS0. The Recombinant Mouse Complement Factor D Protein has been validated for the following applications: Enzyme Activity.

Specifications

• Accession Number: P03953
• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 1675
• Molecular Weight (g/mol): Observed M.W.: 40-49kDa, reducing conditions, Theoretical M.W.: 27kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse Complement Factor D/Adipsin protein Ile26-Ser259,with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Gene Alias: Adipsin, ADN, ADNcomplement factor D, AMBP-1, C3 convertase activator, CFD, complement factor D (adipsin), complement factor D preproprotein, D component of complement (adipsin), DF, EC 3.4.21, EC 3.4.21.46, PFD, Properdin factor DADIPSIN
• Common Name: Complement Factor D/Adipsin
• Biological Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >18 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Ile26-Ser259, with a C-terminal 10-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining