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R&D Systems™ Recombinant Mouse Complement Factor D Protein

Catalog No. 9207SE050
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92-07S-E050 50 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse Complement Factor D Protein is derived from NS0. The Recombinant Mouse Complement Factor D Protein has been validated for the following applications: Enzyme Activity.

Specifications

Accession Number P03953
For Use With (Application) Enzyme Activity
Gene ID (Entrez) 1675
Molecular Weight (g/mol) Observed M.W.: 40-49kDa, reducing conditions, Theoretical M.W.: 27kDa
Quantity 50 μg
Source Mouse myeloma cell line,NS0-derived mouse Complement Factor D/Adipsin protein Ile26-Ser259,with a C-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU per 1μg of the protein by the LAL method.
Gene Alias Adipsin, ADN, ADNcomplement factor D, AMBP-1, C3 convertase activator, CFD, complement factor D (adipsin), complement factor D preproprotein, D component of complement (adipsin), DF, EC 3.4.21, EC 3.4.21.46, PFD, Properdin factor DADIPSIN
Common Name Complement Factor D/Adipsin
Biological Activity Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >18 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived Ile26-Ser259, with a C-terminal 10-His tag
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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