R&D Systems™ Recombinant Mouse CRTAM Fc Chimera Protein

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding activity in a functional ELISA. Galiberet, L. et al. (2005) J. Biol. Chem. 280:21955. Immobilized rmCRTAM/Fc Chimera at 2 μg/mL (100 μL/well) can bind rmIGSF4A with a linear range of 0.15-10 μg/mL.

Specifications

• Accession Number: Q149L7
• For Use With (Application): Binding Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 56253
• Molecular Weight (g/mol): M.W. (Observed): 90 kDa, reducing conditions; M.W. (theoretical): 56.1 kDa (monomer)
• Name: CRTAM Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse CRTAM protein Mouse CRTAM (Ala17 - Ser284) (N-terminus) Accession #Q149L7 IEGRMD Human IgG1 (Pro100 - Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: CRTAM