R&D Systems™ Recombinant Mouse ECE-1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005 ). The specific activity is >2,250 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q4PZA2
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2.
• Gene ID (Entrez): 1889
• Molecular Weight (g/mol): M.W. (Observed): 90-130 kDa, reducing conditions; M.W. (theoretical): 78 kDa
• Name: ECE-1
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse ECE-1 protein Gln89-Trp769, with and N-terminal 6-His tag, Gln89-Trp769
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Structural Form: Disulfide-linked dimer
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: ECE-1