R&D Systems™ Recombinant Mouse EGF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.

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Specifications

• Accession Number: NP_034243
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): M.W. (theoretical): 6.2 kDa
• Name: EGF
• Quantity: 200 μg
• Source: E. coli-derived mouse EGF protein Asn977-Arg1029, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Egf
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: EGF