R&D Systems™ Recombinant Mouse GDF-3 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse GDF-3 Protein is derived from CHO. The Recombinant Mouse GDF-3 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q07104
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 9573
• Molecular Weight (g/mol): Observed M.W.: 14-23kDa, reducing conditions, Theoretical M.W.: 13kDa
• Quantity: 10μg
• Source: Chinese Hamster Ovary cell line,CHO-derived mouse GDF-3 protein Ala253-Gly366
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: GDF3, GDF-3, growth differentiation factor 3, growth/differentiation factor 3, KFS3, MCOP7, MCOPCB6, Vgr-2
• Common Name: GDF-3
• Gene Symbol: Gdf3
• Biological Activity: Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653. 50-100ng/mL of Recombinant Mouse GDF-3 can effectively induce Smad2 phosphorylation.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Ala253-Gly366
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining