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R&D Systems™ Recombinant Mouse GITR Ligand/TNFSF18 (aa 47-173)

Catalog No. 2177GL025
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Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Lyophilized from a 0.2 μm filtered solution in PBS.
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Catalog No. Formulation
2177GL025 Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
2177GL025CF Lyophilized from a 0.2 μm filtered solution in PBS.
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 0.02-0.3 μg/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).

Specifications

Accession Number AAO89011
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Gene ID (Entrez) 8995
Molecular Weight (g/mol) M.W. (Observed): 16-26 kDa, reducing conditions; M.W. (theoretical): 16 kDa
Name GITR Ligand/TNFSF18 aa 47-173
Quantity 25 μg
Source Mouse myeloma cell line, NS0-derived mouse GITR Ligand/TNFSF18 protein Thr47-Ser173, with an N-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias Activation-inducible TNF-related ligand, AITRL, AITRLMGC138237, GITRL, GITRLglucocorticoid-induced TNFR-related protein ligand, Glucocorticoid-induced TNF-related ligand, hGITRLGITR ligand, TL6AITR ligand, TNFSF18, tumor necrosis factor (ligand) superfamily, member 18, tumor necrosis factor ligand superfamily member 18
Gene Symbol Tnfsf18
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Protein GITR Ligand/TNFSF18
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