R&D Systems™ Recombinant Mouse IFN-alpha 2/IFNA2 Protein

Description

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons

• IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits
• The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively
• Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3)
• IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity

Specifications

• Accession Number: P01573
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3440
• Molecular Weight (g/mol): 19.3 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein Cys24-Glu190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after opening. 3 months, -20 to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS