R&D Systems™ Recombinant Mouse IL-10 (HEK293-Expressed) Protein, CF

Description

Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.0650-0.780 ng/mL.

Specifications

• Accession Number: P18893.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3586
• Molecular Weight (g/mol): M.W. (Observed): 16-21 kDa, under reducing conditions., M.W. (Theroretical): 19 kDa
• Quantity: 100 μg
• Source: Human embryonic kidney cell, HEK293-derived mouse IL-10 protein Gln22-Ser178
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CSIFMGC126450, Cytokine synthesis inhibitory factor, IL10A, IL-10MGC126451, interleukin 10, interleukin-10, TGIF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-10