R&D Systems™ Recombinant Mouse IL-12 (linked heterodimer) Protein

Description

GS Linker, Defined heterodimer stoichiometry.

Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.

Specifications

• Accession Number: P43432, P43431
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3592
• Molecular Weight (g/mol): 58 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 10 μg
• Source:

  Mouse myeloma cell line, NS0-derived mouse IL-12 protein

  Human IL-12 p40 (Met23-Ser335) Accession # P43432GGGGSGGGGSGGGGSMouse IL-12 p35 (Arg23-Ala215) Accession # P43431N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS