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R&D Systems™ Recombinant Mouse IL-23 (HEK293-expressed) Protein

Catalog No. 11269ML010
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11-269-ML010 10 μg
11-269-ML050 50 μg
11-269-ML250 250 μg
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Description

Analyzed by SEC-MALS.

Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.050-0.500 ng/mL.

Specifications

Accession Number P43432.1 (p40) & Q9EQ14.1 (p19)
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Weight (g/mol) MolecularWeight-observed: 60-75 kDa, under reducing conditions., MolecularWeight-theroretical: 57 kDa
Quantity 10 μg
Source Human embryonic kidney cell, HEK293-derived mouse IL-23 protein Mouse IL-12p40 (Met22-Ser335) Accession # P43432.1 GSGSSRGGSGSGGSGGGGSKL Mouse IL-23p19 (Leu20-Ala196) Accession # Q9EQ14.1 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
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