R&D Systems™ Recombinant Mouse LIF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Mouse LIF Protein is derived from E. coli. The Recombinant Mouse LIF Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P09056
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3976
• Molecular Weight (g/mol): Observed M.W.: 20kDa, reducing conditions, Theoretical M.W.: 20kDa
• Quantity: 25 μg
• Source: E. coli-derived mouse LIF protein Pro25-Phe203
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor), Melanoma-derived LPL inhibitor, MLPLI
• Common Name: LIF
• Gene Symbol: Lif
• Biological Activity: Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.6ng/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: E. coli-derived Pro25-Phe203
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining