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R&D Systems™ Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein

Catalog No. 8580MG100
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100 μg
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8580MG100 100 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein is derived from NS0. The Recombinant Mouse MAG/Siglec-4a Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

For Use With (Application) Bioactivity
Gene ID (Entrez) 4099
Molecular Weight (g/mol) Observed M.W.: 104-124kDa, reducing conditions, Theoretical M.W.: 82kDa (monomer)
Quantity 100 μg
Source Mouse myeloma cell line,NS0-derived mouse MAG/Siglec-4a protein, (N-terminus) Mouse MAG/Siglec-4a (Gly20-Pro516) Accession &Num; P20917 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias GMAsialic acid binding Ig-like lectin 4A, myelin associated glycoprotein, myelin-associated glycoprotein, sialic acid-binding immunoglobulin-like lectin 4A, Siglec4a, SIGLEC-4A, S-MAG
Common Name MAG/Siglec-4a
Gene Symbol Mag
Biological Activity Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. When immobilized as a 3μL droplet containing 200 ng on a nitrocellulose coated microplate, recombinant mouse MAF Fc Chimera is able to significantly inhibit neurite outgrowth.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived Mouse MAG/Siglec-4a (Gly20-Pro516) Accession # P20917, N-terminus; IEGRMDP; Mouse IgG2a (Glu98-Lys330), C-terminus
Purity or Quality Grade >95%, by SDS-PAGE with Silver Staining
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