R&D Systems™ Recombinant Mouse MMP-12 Protein, CF

Catalog No. 34-67M-PB020

Supplier: R&D Systems™ 3467MPB020

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010 ). The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: EDL24933
• Characterization: Enzyme Activity
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, CHAPS, ZnSO4 and PEG.
• Gene ID (Entrez): 4321
• Molecular Weight (g/mol): M.W. (Observed): 50-62 kDa, reducing conditions; M.W. (theoretical): 52 kDa
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived mouse MMP-12 protein Ala18-Cys462
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 3.4.24, hME, HMEEC 3.4.24.65, Macrophage elastase, macrophage metalloelastase, matrix metallopeptidase 12 (macrophage elastase), matrix metalloproteinase 12 (macrophage elastase), Matrix metalloproteinase-12, ME,mgC138506, MME, MMP12, MMP-12
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 0.25 mg/mL in 10 mM MES, 0.1 M NaCl, 100 μM CaCl2, 0.1% CHAPS, 1 μM ZnSO4 and 0.1% PEG, pH 6.0
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: MMP-12