R&D Systems™ Recombinant Mouse Nephrin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells. When 5 x 104 cells/well are added to rmNephrin coated plates (10 μg/mL, 100 μL/well), >60% will adhere after 90 minutes at 37° C.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS.
• Gene ID (Entrez): 54631
• Molecular Weight (g/mol): 110 kDa
• Name: Nephrin
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse Nephrin protein Gln37-Thr1049,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 85%, by SDS-PAGE under reducing conditions and visualized by silver stain.