R&D Systems™ Recombinant Mouse Noggin (aa 28-232) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-GP ).

Specifications

• Accession Number: P97466
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): M.W. (Observed): 25-35 kDa, reducing conditions; M.W. (theoretical): 23 kDa (monomer)
• Name: Noggin (aa 28-232)
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Noggin protein Gln28-Cys232
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: NOG, noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A
• Gene Symbol: Nog
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Noggin