R&D Systems™ Recombinant Mouse Noggin Fc Chimera Protein

Description

New rm Noggin is Now Available. It has ∼2 fold better activity, lower endotoxin specifications, and no tag.

• Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
• ED50 for this effect is 0.06 to 0.3 μg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4

Specifications

• Accession Number: U79163
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): 50 kDa (monomer)
• Name: NOG, noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A
• Quantity: 10 μg
• Source:

  Mouse myeloma cell line, NS0-derived mouse Noggin protein

  Mouse Noggin (Leu20-Cys232) Accession # U79163IEGRMDHuman IgG1 (Pro100-Lys330)N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: Nog
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.