R&D Systems™ Recombinant Mouse Noggin Protein

Description

∼2 fold better activity; Lower endotoxin specification; Enhanced purity.

• Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
• The ED50 for this effect is 0.06 to 0.3 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.

Specifications

• Accession Number: P97466
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): 26 kDa (monomer)
• Name: NOG, noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A
• Quantity: 100 μg
• Source:

  Mouse myeloma cell line, NS0-derived mouse Noggin protein

  Mouse Noggin (Leu20-Cys232) Accession # P97466IEGRGGGSGGGSGGGS10-His tagN-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: Nog
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.