R&D Systems™ Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is 3-15 ng/mL in the presence of 12 ng/mL of cross-linked Recombinant Human TRAIL/TNFSF10 (Catalog #s 375-TL and MAB050 ).

Specifications

• Accession Number: Q6PI12
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 4982
• Molecular Weight (g/mol): M.W. (Observed): 85-95 kDa, reducing conditions; M.W. (theoretical): 70.9 kDa (monomer)
• Name: Osteoprotegerin/TNFRSF11B Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Osteoprotegerin/TNFRSF11B protein Mouse OPG Glu22-Leu401 (Gln138Arg) (N-terminus) Accession # Q6PI12 IEGRMD Human IgG1 (Pro100-Lys330) (Thr276Ala) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Tnfrsf11b
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Osteoprotegerin/TNFRSF11B