R&D Systems™ Recombinant Mouse PDGF-AB Protein

Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 15.0-75.0 ng/mL.

Specifications

• Accession Number: P20033.2 (A chain) & P31240.1 (B chain)
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 5154
• Molecular Weight (g/mol): M.W.-observed: 17.5 kDa (A chain) & 12.5 kDa (B chain), under reducing conditions., M.W.-theroretical: 14 kDa (A chain) & 12 kDa (beta chain)
• Quantity: 10 μg
• Source: E. coli-derived mouse PDGF-AB protein Mouse PDGF-A (Ser87-Thr211) (N-terminus)Accession # P20033.2 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PDGF-AB