R&D Systems™ Recombinant Mouse Pro-EGF (aa 29-1029) Protein

Catalog No.	Formulation
4095EG025CF	Lyophilized from a 0.2 μm filtered solution in PBS.
4095EG025	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Catalog No. 4095EG025CF Supplier R&D Systems™ Supplier No. 4095EG025/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 1-5 ng/mL.

Specifications

• Accession Number: P01132
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): M.W. (Observed): 160 kDa, reducing conditions; M.W. (theoretical): 110 kDa
• Name: Pro EGF aa 29-1029
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived mouse EGF protein Trp29-Arg1029, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Egf
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: EGF