R&D Systems™ Recombinant Mouse S100A8/S100A9 Heterodimer Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Mouse S100A8/S100A9 Heterodimer Protein is derived from E. coli. The Recombinant Mouse S100A8/S100A9 Heterodimer Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 7kDa and 12kDa, reducing conditions, Theoretical M.W.: 10kDa (S100A8), 13kDa (S100A9)
• Quantity: 50 μg
• Source: E. coli-derived mouse S100A8/S100A9 Heterodimer protein, (N-terminus) Mouse S100A8 (Pro2-Glu89) Accession &Num; P27005 Mouse S100A9 (Ala2-Lys113) Accession &Num; P31725 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: S100A8/S100A9 Heterodimer
• Biological Activity: Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172. The ED50 for this effect is typically 1.5-6μg/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: E. coli-derived Mouse S100A8 (Pro2-Glu89) Accession # P27005, N-terminal; Mouse S100A9 (Ala2-Lys113) Accession # P31725, C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining