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R&D Systems™ Recombinant Mouse Semaphorin 4B Fc Chimera Protein

Catalog No. 10263S4050
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10-263-S4050 50 μg
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Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Semaphorin 4B Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Specifications

Accession Number Q62179.2
For Use With (Application) Bioactivity
Gene ID (Entrez) 10509
Molecular Weight (g/mol) 102 kDa
Name KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B, MGC131831, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B, SEMA4B, SEMAC, semaphorin-4B, SemC
Quantity 50 μg
Source

Mouse myeloma cell line, NS0-derived mouse Semaphorin 4B protein

Mouse Semaphorin 4B (Leu31-Glu703) Accession # Q62179.2IEGRMDPMouse IgG2a (Glu98-Lys330)N-terminus C-terminus

Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Conjugate Unconjugated
Species Mouse
Reconstitution Reconstitute at 1 mg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS.
Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method.
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
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