R&D Systems™ Recombinant Mouse Semaphorin 4B Fc Chimera Protein

Description

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Semaphorin 4B Fc Chimera, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Specifications

• Accession Number: Q62179.2
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 10509
• Molecular Weight (g/mol): 102 kDa
• Name: KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B, MGC131831, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B, SEMA4B, SEMAC, semaphorin-4B, SemC
• Quantity: 50 μg
• Source:

  Mouse myeloma cell line, NS0-derived mouse Semaphorin 4B protein

  Mouse Semaphorin 4B (Leu31-Glu703) Accession # Q62179.2IEGRMDPMouse IgG2a (Glu98-Lys330)N-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Species: Mouse
• Reconstitution: Reconstitute at 1 mg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.