R&D Systems™ Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein

Catalog No.	Quantity
52-35S-4B050	50 μg

Catalog No. 52-35S-4B050 Supplier R&D Systems™ Supplier No. 5235S4B050

Description

Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 (Catalog # 5329-PB ) is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.

Specifications

• Accession Number: NP_038688.2
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 10507
• Molecular Weight (g/mol): M.W. (Observed): 105-135 kDa, under reducing conditions; M.W. (theoretical): 103 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 4 D/CD100 protein Mouse Semaphorin 4 D/CD100 (Phe24-Met711) (N-terminus) Accession # NP_038688.2 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Semaphorin 4 D/CD100