R&D Systems™ Recombinant Mouse Semaphorin 6A Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse Semaphorin 6A Fc Chimera Protein is derived from Sf 21 (stably transfected). The Recombinant Mouse Semaphorin 6A Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 57556
• Molecular Weight (g/mol): Observed M.W.: 94-120kDa, reducing conditions, Theoretical M.W.: 97kDa
• Quantity: 50 μg
• Source: Spodoptera frugiperda,Sf 21 (stably transfected)-derived mouse Semaphorin 6A protein, (N-terminus) Mouse Sema 6A (Gly19-Thr649) Accession &Num; O35464 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: KIAA1368HT018, SEMA, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A, Sema VIA, Sema6A, SEMA6A1, SEMA6A-1, semaphorin 6A-1, Semaphorin VIA, semaphorin-6A, Semaphorin-6A-1, SEMAQ, VIA
• Common Name: Semaphorin 6A
• Biological Activity: Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is typically 0.2-1.2μg/mL.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Spodoptera frugiperda, Sf 21 (stably transfected)-derived Mouse Sema 6A (Gly19-Thr649) Accession # O35464, N-terminus; IEGRMD; Human IgG1 (Pro100-Lys330), C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining