R&D Systems™ Recombinant Mouse sFRP-1 Protein

Catalog No.
90-19S-F025	25μg

Catalog No. 90-19S-F025 Supplier R&D Systems™ Supplier No. 9019SF025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Mouse sFRP-1 Protein is derived from HEK293. The Recombinant Mouse sFRP-1 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q8C4U3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in NaH 2 PO 4 and NaCl with BSA as a carrier protein.
• Gene ID (Entrez): 6422
• Molecular Weight (g/mol): Observed M.W.: 37-43kDa, reducing conditions, Theoretical M.W.: 33kDa
• Quantity: 25μg
• Source: Human embryonic kidney cell,HEK293-derived mouse sFRP-1 protein Ser32-Lys314
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: FRP1, FRP-1FrzA, FRPSARP-2, FrzA, SARP-2, SARP2sFRP-1, Secreted apoptosis-related protein 2, secreted frizzled-related protein 1, sFRP1
• Common Name: sFRP-1
• Gene Symbol: Sfrp1
• Biological Activity: Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 150-900ng/mL in the presence of 300ng/mL of Recombinant Mouse Wnt-3a.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Ser32-Lys314
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining