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R&D Systems™ Recombinant Mouse ST3GAL5/GM3 Synthase Protein

Catalog No. 8454GT050
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50 μg
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84-54G-T050 50 μg
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Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to alpha-lactose. The specific activity is >5 pmol/min/μg, as measured under the described conditions.

Specifications

Accession Number O88829
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Gene ID (Entrez) 8869
Molecular Weight (g/mol) M.W. (Observed): 45-60 kDa, reducing conditions; M.W. (theoretical): 38 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived mouse ST3GAL5/GM3 Synthase protein Thr87-His414, with an N-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Endotoxin Concentration <0.1 EU / 1 μg of the protein by the LAL method.
Gene Alias 3S-T, alpha 2,3-sialyltransferase V, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, EC 2.4.99.9, Ganglioside GM3 synthase, GM3 synthase, lactosylceramide alpha-2,3-sialyltransferase, SATI, Sialyltransferase 9, sialyltransferase 9 (CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase;GM3 synthase), Siat9, SIATGM3S, ST3 beta-galactoside alpha-2,3-sialyltransferase 5, ST3Gal V, ST3GALV, ST3GalVSIAT9SATI
Common Name ST3GAL5
Gene Symbol St3gal5
Biological Activity Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to alpha-lactose. The specific activity is >5 pmol/min/μg, as measured under the described conditions.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived Thr87-His414, with an N-terminal 6-His tag
Purity or Quality Grade >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
Protein ST3GAL5
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