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R&D Systems™ Recombinant Mouse TSH alpha/beta Heterodimer Protein

Catalog No. 8885TH010CF
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8885TH010CF 10 μg
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Catalog No. 8885TH010CF Supplier R&D Systems™ Supplier No. 8885TH010/CF
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse TSH alpha/beta Heterodimer Protein is derived from CHO. The Recombinant Mouse TSH alpha/beta Heterodimer Protein has been validated for the following applications: Bioactivity.

Specifications

For Use With (Application) Bioactivity
Gene ID (Entrez) 7252
Molecular Weight (g/mol) Observed M.W.: 35-41kDa, reducing conditions, Theoretical M.W.: 25kDa
Quantity 10 μg
Source Chinese Hamster Ovary cell line,CHO-derived mouse TSH alpha/beta Heterodimer protein, (N-terminus) Mouse TSH beta (Phe21-Val138) Accession &Num; P12656 (GGGS)3 Mouse TSH alpha (Leu25-Ser120) Accession &Num; P01216 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Common Name TSH alpha/beta
Biological Activity Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human TSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The ED50 for this effect is typically 0.015-0.15ng/mL.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived Mouse TSH beta (Phe21-Val138) Accession # P12656, N-terminus; (GGGS)3; Mouse TSH alpha (Leu25-Ser120) Accession # P01216, C-terminus
Purity or Quality Grade >95%, by SDS-PAGE with Silver Staining
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