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R&D Systems™ Recombinant Porcine PD-L1/B7-H1 Fc Chimera Protein

Catalog No. 10397B7100
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100 μg
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10-397-B7100 100 μg
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Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA. When Recombinant Porcine PD-L1/B7-H1 Fc Chimera (Catalog # 10397-B7) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Porcine PD-1 His-tag that produces 50% of the optimal binding response is found to be approximately 0.8-6 μg/mL.

Specifications

Accession Number NP_001020392
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 29126
Molecular Weight (g/mol) M.W. (Observed): 65-85 kDa, under reducing conditions; M.W. (theoretical): 52 kDa
Quantity 100 μg
Source Chinese Hamster Ovary cell line, CHO-derived porcine PD-L1/B7-H1 protein Porcine PD-L1/B7-H1 (Phe19-Thr237) (N-terminus) Accession # NP_001020392.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PD-L1
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