R&D Systems™ Recombinant Porcine PD-L1/B7-H1 His-tag Protein

Description

Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA. When Recombinant Porcine PD-L1/B7-H1 His-tag (Catalog # 10361-B7) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD ) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 μg/mL.

Specifications

• Accession Number: NP_001020392.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 29126
• Molecular Weight (g/mol): M.W. (Observed): 35-55 kDa, under reducing conditions; M.W. (theoretical): 26 kDa
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived porcine PD-L1/B7-H1 protein Phe19-Thr237, with a C-terminal 6-His tag.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-L1