R&D Systems™ Recombinant Rat B7-H2 Fc Chimera Protein

Catalog No.	Quantity
10-419-B7100	100 μg

Catalog No. 10-419-B7100 Supplier R&D Systems™ Supplier No. 10419B7100

Description

Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA. When Recombinant Rat B7-H2 Fc Chimera (Catalog # 10419-B7) is Immobilized at 1.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS ) that produces 50% optimal binding response is found to be approximately 10-100 ng/mL.

Specifications

• Accession Number: XP_006256322.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 23308
• Molecular Weight (g/mol): M.W. (Observed): 70-90 kDa, under reducing conditions; M.W. (theoretical): 54 kDa
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived rat B7-H2 protein Rat B7-H2 (Glu25-Lys261) (N-terminus) Accession # XP_006256322.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H2/ICOSLG