R&D Systems™ Recombinant Rat CD40 Ligand/TNFSF5 (HA-tag) Protein

Catalog No.	Quantity
84-14C-L025CF	25 μg

Catalog No. 84-14C-L025CF Supplier R&D Systems™ Supplier No. 8414CL025/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is 0.15-0.9 ng/mL in the presence of 0.1 μg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).

Specifications

• Accession Number: Q9Z2V2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in sterile PBS and NaCl.
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): M.W. (Observed): 22 - 30 kDa, reducing conditions; M.W. (theoretical): 22 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived rat CD40 Ligand/TNFSF5 protein HA (YPYDVPDYA) GCN4-IZ GGGSGGGSGGGS Rat CD40 Ligand (Met112-Leu260 (N-terminus) Accession # Q9Z2V2 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
• Common Name: CD40 Ligand/TNFSF5
• Gene Symbol: Cd40lg
• Biological Activity: Measured in a cell proliferation assay using mouse splenic B cells in the presence of IL-4. Banchereau, J. et al. (1991) Science 251:70. The ED50 for this effect is typically 0.15-0.9ng/mL in the presence of 0.1μg/mL of the cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.
• Conjugate: Unconjugated
• Species: Rat
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Expression System: Chinese Hamster Ovary cell line, CHO-derived HA (YPYDVPDYA), N-terminus; GCN4-IZ; GGGSGGGSGGGS; Rat CD40 Ligand (Met112-Leu260 Accession # Q9Z2V2, C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: CD40 Ligand/TNFSF5