R&D Systems™ Recombinant Rat PD-1 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7 ) is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) that produces 50% optimal binding response is typically 0.1-0.6 μg/mL.

Specifications

• Accession Number: NP_001100397
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with Sucrose and Mannitol.
• Gene ID (Entrez): 5133
• Molecular Weight (g/mol): M.W. (Observed): 57-70 kDa, reducing conditions; M.W. (theoretical): 43 kDa
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived rat PD-1 protein Rat PD-1 (Leu25-Gln167) (N-terminus) Accession # NP_001100397 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Storage Requirements: 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
• Specific Reactivity: PD-1
• Conjugate: Unconjugated
• Species: Rat
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL aq. soln..
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with Sucrose and Mannitol.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PD-1