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R&D Systems™ Recombinant Rat PD-L1/B7-H1 Fc Chimera Protein

Catalog No. 9835B7050
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98-35B-7050 50 μg
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Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD ) at 1 μg/mL (100 μL/well) can bind Recombinant Rat PD-L1/B7-H1 Fc Chimera with an ED50 of 0.09-0.54 μg/mL.

Specifications

Accession Number NP_001178883
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 29126
Molecular Weight (g/mol) M.W. (Observed): 74-84 kDa, reducing conditions; M.W. (theoretical): 52 kDa
Quantity 50 μg
Source Mouse myeloma cell line, NS0-derived rat PD-L1/B7-H1 protein Rat B7-H1 (Phe19-Thr238) (N-terminus) Accession # NP_001178883 IEGRMDP Mouse IgG2a (Glu98-Lys330) (C-terminus)
Storage Requirements 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Unconjugated
Species Rat
Reconstitution Reconstitute at 500 μg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein PD-L1
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