Novus Biologicals™ Recombinant Rat TNF-alpha Protein

Catalog No.	Quantity
NB805766	5 μg
NB804779	1 mg
NB805653	100 μg
NB805362	500 μg

Catalog No. NB805766 Supplier Novus Biologicals™ Supplier No. NBP2353005UG

Description

Recombinant Protein

TNF alpha Protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 x 10^7 IU/mg in the presence of actinomycin D.

Specifications

• Accession Number: P16599, P16599, P16599
• For Use With (Application): SDS-PAGE, Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2, 150 mM NaCl.
• Gene ID (Entrez): 7124
• Molecular Weight (g/mol): MolecularWeight-theroretical: 17.2 kDa
• Quantity: 5 μg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.)
• Endotoxin Concentration: Less than 1 EU/μg of TNF-alpha as determined by LAL method.
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alpha, TNF-alpha/TNFSF2, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Gene Symbol: TNF
• Reconstitution: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL Apportion stock solutions into working aliquots and store (at <-20°C.)
• Research Category: Adaptive Immunity, Asthma, Autoimmune Diseases, Autophagy, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Purity or Quality Grade: >98 % pure by SDS-PAGE and HPLC
• Protein: TNF-alpha