R&D Systems™ Recombinant SARS-CoV2 B.1.617.2 RBD Alexa Fluor™ 488 Protein

Description

His-tag Delta Variant (India) L452R T478K

Measured by flow cytometry for its ability to bind HEK293 human embryonic kidney cells transfected with human ACE-2 at 0.250-1.00 μg/mL (100 μL/well).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: YP_009724390.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Molecular Weight (g/mol): M.W.-observed: 34-38 kDa, under reducing conditions., M.W.-theroretical: 26 kDa
• Quantity: 20 μg
• Source: Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike RBD protein Arg319-Phe541 (Leu452Arg, Thr478Lys), with a C-terminal 6-His tag
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Alexa Fluor 488
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Spike RBD