R&D Systems™ Recombinant TEV Protease Protein

Catalog No.	Quantity
4469TP200	200 μg

Catalog No. 4469TP200 Supplier R&D Systems™ Supplier No. 4469TP200

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln, with the cleavage point after Gln. TEV Protease cleaves =50% of the control substrate, as measured under the described conditions. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.

Specifications

• Accession Number: NP_062908
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.
• Gene ID (Entrez): 1502321
• Molecular Weight (g/mol): M.W. (Observed): 25-30 kDa, reducing conditions; M.W. (theoretical): 28.5 kDa
• Name: Sleeping Beauty Transposase
• Quantity: 200 μg
• Source: E. coli-derived viral TEV Protease protein Ala TEV Protease Glu2039-Gln2279 (Ser2256Asn) (N-terminus) Accession # NP_062908 Leu 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: NIa, P1 Protease, Tobacco Etch Virus Protease
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: TEV Protease