R&D Systems™ Recombinant Viral 136R/Y136 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Viral 136R/Y136 Protein is derived from CHO. The Recombinant Viral 136R/Y136 Protein has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 88-120kDa, reducing conditions, Theoretical M.W.: 41kDa
• Quantity: 25μg
• Source: Chinese Hamster Ovary cell line,CHO-derived viral 136R protein, (N-terminus) Viral 136R/Y136 (Tyr17-Lys351) Accession &Num; Q9DHH7 HPGGGSGGGSGGGS HHHHHH (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: 136R
• Biological Activity: Measured by its ability to inhibit Type-I and Type-III IFN-mediated anti-viral activity. Huang, J. et al. (2007) PANS 104:9822. The ED50 for this effect is typically 2-12ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta.
• Conjugate: Unconjugated
• Species: Virus
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Viral 136R/Y136 (Tyr17-Lys351) Accession # Q9DHH7, N-terminus; HPGGGSGGGSGGGS; HHHHHH, C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining