PeproTech RELM beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No.	Quantity
50-271-8602	500 μg

Catalog No. 50-271-8602 Supplier PeproTech Supplier No. 500P217BT500UG

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human RELMbeta. Anti-Human RELMbeta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human RELMbeta (500-P217) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human RELMbeta. Western Blot: To detect Human RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human RELMbeta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P217BT-1MG will be provided as 2 x 500 μg

Adipose tissue is an energy reserve that is strictly regulated in animals. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

Specifications

• Antigen: RELM beta
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Biotin
• Formulation: PBS with no preservative
• Gene: Retnlb
• Gene Accession No.: Q9BQ08
• Gene Alias: 9030012B21Rik; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2; CCRG; Colon and small intestine-specific cysteine-rich protein; colon carcinoma-related gene protein; cysteine-rich secreted A12-alpha-like protein 1; cysteine-rich secreted protein A12-alpha-like 1; Cysteine-rich secreted protein A12-beta; cysteine-rich secreted protein FIZZ2; FIZZ1; Fizz2; found in inflammatory zone 1; found in inflammatory zone 2; HXCP2; Relmb; RELMbeta; RELM-beta; resistin like beta; resistin-like beta; resistin-like molecule beta; RETNL2; RETNLB; RSNB; UNQ408/PRO770; XCP2; Xcp3
• Gene Symbols: Retnlb
• Host Species: Rabbit
• Immunogen: E.coli-derived, 19.0 kDa Recombinant Human RELMbeta.
• Purification Method: Antigen affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 84666
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG