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Invitrogen™ Renilla luciferase Polyclonal Antibody
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Catalog No. PIPA532210
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100 μL
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PIPA532210 100 μL
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Catalog No. PIPA532210 Supplier Invitrogen™ Supplier No. PA532210
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Description

Rabbit Polyclonal Antibody

Recommended positive controls: 293T transfected Renilla Luciferase. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Renilla luciferase (RLuc), derived from the sea pansy Renilla reniformis, is a widely-used reporter gene in molecular biology for in vitro and in vivo assays, including gene expression studies and small animal imaging. The luciferase enzyme, approximately 37 kDa in size, belongs to the alpha/beta hydrolase family and operates via a catalytic mechanism involving the oxidative decarboxylation of its substrate, coelenterazine, in the presence of molecular oxygen, resulting in the emission of blue light. The gene encoding Renilla luciferase has been isolated and characterized, with its cDNA available for various applications. Structurally, RLuc contains a hydrolase fold akin to that of bacterial dehalogenases, suggesting an evolutionary relationship. Despite extensive utilization, the exact details of its active site and catalytic mechanism continue to be explored. The bioluminescent property of RLuc is particularly useful for generating a detectable signal in various biological assays, enhancing its utility as a biosensor.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Renilla luciferase
Applications Immunohistochemistry (Free Floating), Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1.14 mg/mL
Conjugate Unconjugated
Formulation PBS with 20% glycerol and 0.025% ProClin 300; pH 7
Gene Alias Renilla-luciferin 2-monooxygenase
Host Species Rabbit
Immunogen Full length Renilla Luciferase recombinant protein.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Luciferase
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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