Biotium Resazurin, sodium salt

Supplier:  Biotium 10054

Resazurin is the N-oxide of the fluorescent dye resorufin and used to detect reductive activities in cells. It is widely used for measuring cell proliferation and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until reduced to the highly red fluorescent resorufin (Ex/Em: 563/587 nm) in the presence of reductants like NADPH or NADH and the enzyme diaphorase. Resazurin can therefore be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin /diaphorase /NADPH system can be used to detect any biochemical or enzyme activity involved in generating NADH or NADPH. Although resazurin is available from several suppliers, trace amounts of the resorufin contaminant often make the material unsuitable for bioassay applications. This high-grade resazurin has a minimal background fluorescence. Properties: Ex/Em (pH 9) = 604 nm/none; Ex/Em (reduced): 563/587 nm; Dark solid soluble in DMSO or water; Store at 4°C protected from light; C12H6NNaO4; MW: 251.17

Catalog No. 50-196-4100