Invitrogen™ Retinol Binding Protein ELISA Kit

Description

ELISA

The Retinol Binding Protein Competitive ELISA quantitates retinol binding protein (RBP) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The Retinol Binding Protein Competitive ELISA research-use-only kit is designed to quantitatively measure RBP independent of species. An RBP standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-sheep IgG antibodies. Anti-sheep RBP antibodies and RBP-peroxidase conjugate is added to the wells. The RBP-peroxidase conjugate and any RBP in the sample will compete to bind to the anti-sheep RBP antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of RBP present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Accession Number: C CRABP-I; cellular retinol binding protein 1; Cellular retinol-binding protein; Cellular retinol-binding protein I; CRABP-I; CRBP; CRBP1; Crbpi; CRBP-I; LOW QUALITY PROTEIN: retinol-binding protein 1; mCRBPI; RBP; Rbp1; Rbp-1; RBPC; retinol; retinol binding; Retinol Binding Protein; retinol binding protein 1; retinol binding protein 1, cellular; retinol-binding protein 1; retinol-binding protein 1, cellular
• Assay Range: 7.8 to 1000 ng/mL
• Assay Sensitivity: 1.36 ng/mL, 1.36 μg/mL, 1.36 μg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 5947
• Gene Symbol: Rbp1
• Interassay CV: 12%
• Intraassay CV: 5
• Kit Contents: Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
• Storage Requirements: -20°C
• Target: RBP1
• Test Time: 45 mins
• Total Assay Time: 1 hrs 30 mins