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RNA Polymerase II, Mouse anti-Human, Yeast, Clone: 8WG16, Millipore Sigma™

Mouse Monoclonal Antibody

$144.10 - $369.00

Specifications

Antigen RNA Polymerase II
Clone 8WG16
Applications ChIP assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Host Species Mouse
Target Species Human, Yeast
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
05952I25UG
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MilliporeSigma
05952I25UG
25μg
Each for $144.10
N/A
05952I100UG
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MilliporeSigma
05952I100UG
100μg
Each for $369.00
N/A
Description

Description

Anti-RNA Polymerase II, CTD, clone 8WG16, Cat. No. 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting.

DNA-directed RNA polymerase II subunit RPB1 (UniProt: P24928; also known as EC: 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, is encoded by the POLR2A (also known as POLR2) gene (Gene ID: 5430) in human. DNA-dependent RNA polymerase II catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RPB1 subunit is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that suggested to grab the incoming DNA template. It has multiple zinc and magnesium ion binding sites and contains a repetitive C-terminal domain (CTD; aa 1593-1960) that consists of tandem repeats of the consensus sequence YSPTSPS and serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. It can exist in both hypophosphorylated form, which is mainly cytosolic, and in hyperphosphorylated form. The phosphorylation of Serine 2 and 5 in the hepatapeptide repeat is achieved by Cdk7 and Cdk9. Cdk7 phosphorylation promotes transcription initiation by triggering promoter release and elongation.
Specifications

Specifications

RNA Polymerase II
ChIP assay, Dot Blot, Immunoprecipitation, Inhibition Assays, Western Blot
Human, Yeast
Primary
8WG16
Mouse
Monoclonal
SDS
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