Invitrogen™ ROBO1 Polyclonal Antibody

Catalog No.	Quantity
PIPA529917	100 μL

Catalog No. PIPA529917 Supplier Invitrogen™ Supplier No. PA529917

Description

Rabbit Polyclonal Antibody

Recommended positive controls: 293T, A431, HeLa, HepG2, MDA-Mb-361, HCC1937 LROBO1 (Lentivirus infection, carry human ROBO1). Predicted reactivity: Mouse (100%), Rat (97%), Zebrafish (80%), Xenopus laevis (95%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

Specifications

• Antigen: ROBO1
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1.26 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: ROBO1
• Gene Accession No.: O89026, Q9Y6N7
• Gene Alias: AW494633; AW742721; deleted in U twenty twenty; DUTT1; FLJ21882; Gm310; HGNC:10249; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout 1; roundabout guidance receptor 1; roundabout homolog 1; roundabout homolog 1 (Drosophila); roundabout, axon guidance receptor, homolog 1; roundabout, axon guidance receptor, homolog 1 (Drosophila); Roundabout1 protein; SAX3
• Gene Symbols: ROBO1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the Extracellular domain of human ROBO1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19876, 395292, 6091
• Target Species: Chicken, Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG